微米在細(xì)胞分離納米

廣泛的細(xì)胞分離技術(shù)本文中描述說(shuō)明了高水平的興趣和活動(dòng)在這一領(lǐng)域。描述的大小和基于密度的方法提供了一個(gè)潛力巨大的分離細(xì)胞亞種群的特定的標(biāo)記不清楚或無(wú)法使用(例如防止細(xì)胞激活)。

基于吸引力的方法(熒光、磁、結(jié)合力和電泳)可以使用快速(~分鐘)和連續(xù)分離高特異性(~ 99%)。的所有方法，設(shè)備的設(shè)計(jì)是這樣的，他們可以在大規(guī)模并行的方式增加規(guī)模和吞吐量的前提下純度和效力。

此外，微流體分離系統(tǒng)可以很容易地整合與單細(xì)胞等設(shè)備，執(zhí)行下游分析裂解和蛋白質(zhì)組和基因組分析。先進(jìn)水平的設(shè)計(jì)、制造和測(cè)量功能，我們預(yù)計(jì)，在未來(lái)幾年重點(diǎn)將從“概念驗(yàn)證”的原型設(shè)備，很容易在經(jīng)濟(jì)生產(chǎn)和應(yīng)用，如即時(shí)臨床診斷方法、藥物發(fā)現(xiàn)，化學(xué)生物學(xué)代理檢測(cè)。

The broad spectrum of cell separation technologies described in this review illustrates the high level of interest and activity in this area. The described size- and density-based approaches offer a great potential for separation of cell subpopulations for which specific markers are not known or cannot be used (eg, to prevent cell activation). Affinity-based approaches (fluorescence-, magnetic-, adhesion-based, and electrophoretic) can be employed for fast (~minutes) and continuous separation with high specificity (~99%). For all of the approaches, the design of the devices is such that they can be operated in a massively parallel fashion to increase scale and throughput without compromising purity and efficacy.